Chemoenzymatic synthesis of branched oligo- and polysaccharides as potential substrates for starch active enzymes |
| |
Authors: | Greffe Lionel Jensen Morten T Bosso Claude Svensson Birte Driguez Hugues |
| |
Affiliation: | Centre de Recherches sur les Macromolécules Végétales (CERMAV-CNRS), Affiliated with Université Joseph Fourier, B.P. 53, 38041 Grenoble Cedex 9, France. |
| |
Abstract: | Oligo- and polysaccharides embodying the alpha-maltotriosyl-6(II)-maltotetraosyl structure were readily synthesized by transglycosylation of maltosyl fluoride onto panose and pullulan catalysed by the bacterial transglycosylase cyclodextrin glycosyltransferase (CGTase). The two products obtained proved useful for increasing the knowledge of substrate binding and processing at the active site of barley limit dextrinase that is involved in the metabolism of amylopectin by acting upon its branch points. |
| |
Keywords: | active site mapping carbohydrates enzyme catalysis glycosyltransferases oligosaccharides |
本文献已被 PubMed 等数据库收录! |