| 亳菊不同部位绿原酸及总黄酮的含量测定 |
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| 引用本文： | 刘劲松,高卫娜,蒋静.亳菊不同部位绿原酸及总黄酮的含量测定[J].广州化工,2014(12):143-145. |
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| 作者姓名： | 刘劲松 高卫娜 蒋静 |
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| 作者单位： | 安徽中医药大学,现代中药安徽省重点实验室,安徽合肥230031 |
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| 基金项目： | 安徽省科学技术厅年度重点科研计划项目（ No1031043016）。 |
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| 摘 要： | 研究亳菊不同部位绿原酸及总黄酮的含量。绿原酸采用Phenomenex C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.4%磷酸水溶液(12∶88)洗脱,检测波长为327 nm;总黄酮采用比色法进行测定,以亚硝酸钠-硝酸铝为显色剂进行测定,检测波长为510 nm。结果表明:亳菊不同部位绿原酸的含量分别是根(0.39%)、茎(0.42%)、叶(0.72%)、花(0.54%);总黄酮的含量分别是根(10.30%)、茎(9.24%)、叶(35.69%)、花(18.08%)。亳菊叶中绿原酸及总黄酮的含量最高。
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| 关 键 词： | 亳菊 不同部位 化学成分 含量 |
Determination of Chlorogenic Acid and Flavonoids Content of Different Parts of Chrysanthemum morifolium Ramat |
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| LIU Jin-song,GAO Wei-na,JIANG Jing.Determination of Chlorogenic Acid and Flavonoids Content of Different Parts of Chrysanthemum morifolium Ramat[J].GuangZhou Chemical Industry and Technology,2014(12):143-145. |
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| Authors: | LIU Jin-song GAO Wei-na JIANG Jing |
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| Affiliation: | (School of Materia Madica, Anhui University of Chinese Medicine, Anhui Key Laboratory of Modernized Chinese Material Medical, Anhui Hefei 230031, China) |
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| Abstract: | The content determination in different parts of Chrysanthemum morifolium Ramat was studied.The content of chlorogenic acid was determined by HPLC on Phenomenex C18 （250 mm ×4.6 mm, 5 μm） column with CH3 CN-0.4%H3 PO4 （ 12：88 ） as mobile phase.The absorbance was monitored at 327 nm.The content of flavonoid was determined by spectrophotometry, with NaNO2 -Al（ NO3 ） 3 as chromogenic agent.The absorbance was monitored at 510 nm.The results showed that the different parts of chlorogenic acid in Chrysanthemum morifolium Ramat was different： root（0.39%） , steam（0.42%） , leave（0.72%） , and flower（0.54%）.The different parts of flavonoid was different： root （ 10.30%） , steam （ 9.24%） , leave （ 35.69%） , and flower （ 18.08%）.The highest level of Chlorogenic acid and flavonoid was in leaves. |
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| Keywords: | Chrysanthemum morifolium Ramat different parts chemical composition content |
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