低温苯胺降解菌的筛选及其特性

通过驯化培养,从含苯胺的管道污泥中分离出1株高效苯胺降解菌7#,在苯胺质量浓度低于5500 mg/L的普通培养基中均可生长。7#菌株降解苯胺的最适温度为30℃,经低温驯化后,10℃和30℃时对苯胺的降解率接近,最适pH=7.0,当苯胺质量浓度在400 mg/L以下时,对该菌不表现出明显抑制作用。在苯胺质量浓度200 mg/L的无机盐溶液中,10℃下培养60 h可使苯胺降解率达89.14%,降解符合二级动力学模型。经鉴定,该菌株为假单胞菌属。

Screening and characteristics of low temperature aniline-degrading strain

Through domestication and culture,a bacterial strain 7#,which was able to grow on common substrate with aniline below 5?500?mg/L,was isolated from the sludge in conduits.The optimum temperature and pH for its growth and aniline degradation were 30℃ and 7.0 respectively,but after low temperature domestication,the degradation rate at 10℃ was close to that at 30℃.The restraint contribution was not be obvious under the concentration of aniline below 400?mg/L.At 10℃,the aniline-degradation rate reached 89.14% when the culture time was 60 hours in solutions with aniline of 200?mg/L and the degradation accorded with the second-order kinetics.Strain 7# was identified as Pseudomonas.