普通小球藻异养-光自养串联培养的培养基

采用单因素实验和均匀实验获得了适合于普通小球藻异养-光自养串联培养的培养基(HA-SK培养基)，其关键是C/N比和保证足够的C, N供应. 采用该培养基在摇瓶中异养-光自养串联培养普通小球藻，异养培养结束时细胞密度达13.17 g/L，经过36 h光自养培养后藻体蛋白质和叶绿素含量分别达49.75%和30.17 mg/g. 用5 L生物反应器和1 L平板光生物反应器串联培养，藻细胞密度最高可达15.36 g/L，藻体蛋白质和叶绿素含量分别达54.78%和31.23 mg/g. 表明采用HA-SK培养基进行异养-光自养串联培养可实现普通小球藻的高密度高品质培养.

Medium for Culturing Chlorella vulgaris with Sequential Heterotrophic-Autotrophic Model

The medium (HA-SK medium) for culturing Chlorella vulgaris with sequential heterotrophic-autotrophic model was optimized by using the one-at-a-time strategy and uniformity experimental design. When Chlorella vulgaris was cultured with HA-SK medium in 250 mL flask, the cell density reached 13.17 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 49.75% and 30.17 mg/g after 36 h autotrophic cultivation, which were increased by 134%, 69.8% and 68.7% respectively comparing with those obtained in an initial medium. As Chlorella vulgaris was cultured with HA-SK medium in sequential 5 L bioreactor and 1 L plat photo-bioreactor system, the cell density reached 15.36 g/L at the end of heterotrophic cultivation, the cellular protein and chlorophyll content were 54.78% and 31.23 mg/g after 35 h autotrophic cultivation. The results show that the cultivation of Chlorella vulgaris with high cell density and high quality can be realized successfully by sequential heterotrophic-autotrophic model with HA-SK medium.