| 高压液相色谱法检测B族维生素及咖啡因的研究 |
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| 引用本文： | 王竹天,杨大进.高压液相色谱法检测B族维生素及咖啡因的研究[J].中国食品卫生杂志,1998(6):9-11. |
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| 作者姓名： | 王竹天 杨大进 |
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| 作者单位： | 卫生部食品卫生监督检验所 |
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| 摘 要： | 用高压液相色谱法检测保健食品中Ｂ族维生素和咖啡因的含量。使用甲醇：水：磷酸＝１００：４００：０．５作提取溶剂，以硫酸月桂酯钠溶液：乙腈：磷酸＝５３０：４７０：１作流动相在２６０ｎｍ波长处检测ＶＢ１，以１－癸烷磺酸钠溶液：乙腈：磷酸＝８５０：１５０：１作流动相在２８０ｎｍ处测ＶＢ６、咖啡因、烟酸、烟酰胺。结果表明该法灵敏度高、特异性强，简便快速、准确
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| 关 键 词： | 维生素B.复合 咖啡因 色谱法.高压液相 食品分析 |
高压液相色谱法检测B族维生素及咖啡因的研究 |
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| Wang Zhutian,Yang Dajin,Fang Congrong et al.高压液相色谱法检测B族维生素及咖啡因的研究[J].Chinese Journal of Food Hygiene,1998(6):9-11. |
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| Authors: | Wang Zhutian Yang Dajin Fang Congrong |
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| Affiliation: | Wang Zhutian Yang Dajin Fang Congrong et al |
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| Abstract: | An HPLC method for determination of Vitamin B and caffeine in health foods was described. Vitamin B1 was detected at 260 nm based on: methyl/distilled water/phosphoric acid(100/400/0.5) as extraction and sodium dodecylsulfate/acetonitrile/phosphoric acid(530/470/1) as mobile phases. Vitamin B6, caffeine, niacin and niacinamide were examined at 280 nm with sodium 1-decanesulfonate/acetonitrile/phosphoric acid(850/150/1) as mobile phase. It is concluded that this method is suitable for rapid and accurate analysis of Vitamin B and caffeine in health foods. Author's address Wang Zhutian, Institute of Food Safety Control and Inspection, Ministry of the Public Health, 100021 PRC. |
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| Keywords: | Vitamin B Complex Coffeine Chromatography High Performance Liquid Food Analysis |
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