| TISTD反应器中的菌群结构及生态变化 |
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| 引用本文： | 叶姜瑜,丁维,何强,孙兴福. TISTD反应器中的菌群结构及生态变化[J]. 土木建筑与环境工程, 2011, 33(1): 147-152 |
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| 作者姓名： | 叶姜瑜 丁维 何强 孙兴福 |
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| 作者单位： | 重庆大学 城市建设与环境工程学院,三峡库区生态环境教育部重点实验室,重庆 400045;重庆大学 城市建设与环境工程学院,三峡库区生态环境教育部重点实验室,重庆 400045;重庆大学 城市建设与环境工程学院,三峡库区生态环境教育部重点实验室,重庆 400045;重庆大学 城市建设与环境工程学院,三峡库区生态环境教育部重点实验室,重庆 400045 |
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| 基金项目： | 科技部国际合作项目(2007DFA90660);国家水体污染控制与治理科技重大专项(2008ZX07315-003) |
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| 摘 要： | 以两相一体式污泥浓缩消化反应器(Two-phase Integrated Sludge Thickening and Digestion reactor,TISTD)为对象,研究了反应器稳定运行期间菌群结构及反应器负荷改变时其内部生态结构的变化.在以30％的投配率稳定运行时,从产酸相和产甲烷相分别取样、培养和分离出20...
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| 关 键 词： | TISTD 菌群结构 温度梯度凝胶电泳 多样性 16S rDNA 生态系统 |
Microbial Community Structure and Ecological Variation in TISTD Reactor |
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| YE Jiang yu,DING Wei,HE Qiang and SUN Xing fu. Microbial Community Structure and Ecological Variation in TISTD Reactor[J]. Journal of Civil,Architectrual & Environment Engineering, 2011, 33(1): 147-152 |
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| Authors: | YE Jiang yu DING Wei HE Qiang SUN Xing fu |
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| Affiliation: | College of Urban Construction and Environmental Engineering, Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing 400045,P.R.China;College of Urban Construction and Environmental Engineering, Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing 400045,P.R.China;College of Urban Construction and Environmental Engineering, Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing 400045,P.R.China;College of Urban Construction and Environmental Engineering, Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing 400045,P.R.China |
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| Abstract: | Based on the sewage treatment device of Two-phase Integrated Sludge Thickening and Digestion reactor (TISTD), microbial community structure in stable operation and its ecological variation when load changes are investigated. During a stable operation with 30% sludge dosage rate, 20 dominant bacterial strains are individually isolated from acid-phase and methane-phase of the reactor. Sequence analysis of 16S rDNA reveals that six of the achieved strains belong to Bacillus, Methanospirillum and Methanococcus respectively. Ten sludge samples are collected separately in 5 periods when the reactor operates with 10%, 20% and 30% sludge dosage rate and in the state of started-up and disordered, and followed by DNA extraction, purification, 16S rDNA PCR amplification and temperature gradient gel electrophoresis (TGGE). The good quality of DNA extraction and amplification demonstrates that there is an abundant biomass in the sludge under mid-temperature condition. The results show that TISTD reactor has a high biodiversity in microbial communities and is complex and stable in ecosystem structure, which effectively supports the good digestion in sludge thickening. |
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| Keywords: | TISTD microbial community structure TGGE diversity 16S rDNA ecosystems |
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