Triacylglycerols of the red microalga Porphyridium cruentum can contribute to the biosynthesis of eukaryotic galactolipids |
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Authors: | Inna Khozin-Goldberg Hu Zheng Yu Daniel Adlerstein Shoshana Didi-Cohen Yair M Heimer Zvi Cohen |
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Affiliation: | (1) Microalgal Biotechnology Laboratory, The Albert Katz Department of Dryland Biotechnologies, Jacob Blaustein Institute for Desert Research, Sde-Boker Campus 84990, Israel;(2) The Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel |
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Abstract: | A mutant of the red microalga Porphyridium cruentum was selected on the basis of impaired growth at suboptimal temperatures (15 vs. 25°C). Fatty acid and lipid analyses revealed
diminished proportions of eicosapentaenoic acid (from 41 to 30%) and of the eukaryotic molecular species (from 38 to 28% of
monogalactosyldiacylglycerol (MGDG) and elevated proportion (10 vs. 2%) of triacylglycerols (TAG) in the mutant, as compared
with the wild type. Pulse labeling of the wild type cells with radioactive fatty acid precursors indicated an initial incorporation
of the fatty acids into phosphatidylcholine (PC) and TAG. Following the pulse, the label of PC and TAG decreased with time
(from 25 to 5% of the total dpm in TAG) while that of chloroplastic polar lipids, mainly MGDG, continued to increase. In the
mutant, however, the labeling of TAG after the pulse was higher (30% of the total dpm) than that of the wild type and decreased
only slightly to 20%. This may indicate that in P. cruentum, TAG can contribute to the biosynthesis of eukaryotic species of MGDG. |
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