脂肪酶水解L-亮氨酸异丁酯的工艺

以脂肪酶A为催化剂,水解L-亮氨酸异丁酯制备L-亮氨酸。研究了不同酶、缓冲液体系、物料比、反应温度、反应时间对L-亮氨酸收率的影响,通过正交试验确定了L-亮氨酸异丁酯最佳水解条件。以L-亮氨酸异丁酯为原料,在提取脂肪酶液为Gly-NaOH缓冲液体系、反应前添加L-亮氨酸达到L-亮氨酸溶解度的质量、物料比为1∶13、50℃、摇床转速为170r/min的条件下反应13h,L-亮氨酸的收率为95.23%。

Research on hydrolysis of L-leucine isobutyl ester by lipase

L-leucine was prepared by hydrolysis of L-leucine isobutyl ester using lipase A as the catalyst.The effects of different enzymes,buffer system,substrate-to-enzyme solution ratio, temperature and reaction time on L-leucine yields were studied in single-factor experiments,and the optimal reaction condition was obtained through orthogonal experimental design.The L-leucine yield reached 95.23% under the optimized reaction condition of Gly/NaOH buffer system, substrate-to-enzyme solution ratio of 1∶13,50℃,shaker rotation speed of 170r/min,and 13h.