利用多重PCR同时检测WSSV和MBV两种对虾病毒的研究

研究了检测斑节对虾(Penaeus monodon)的主要致病病原——对虾白斑综合征病毒(white spot syndrome virus，WSSV)及斑节对虾杆状病毒(monodon baculovirus，MBV)的技术。用多重PCR检测方法，设计了两对特异性引物，从不同虾池中收集斑节对虾，提取DNA模板，同时检测两种对虾病毒。研究结果表明：该方法检测灵敏度高、特异性好，可检测至每毫克组织100个病毒粒子；从对虾组织中提取的DNA模板对病毒DNA的扩增无抑制，适合于对虾中两种病毒的同时检测。

Simultaneous detection of white spot syndrome virus (WSSV) and monodon baculovirus (MBV) by multiplex polymerase chain reaction (PCR) in Penaeus monodon

A method of simultaneously detecting white spot syndrome virus (WSSV) and monodon baculovirus (MBV) in Penaeus monodon was developed by using a single-tube, one-step multiplex polymerase chain reaction (PCR) was established. Two primer sets, W 1/W 2 and M 1/M 2 were mixed to amplify specific fragments of the WSSV and MBV, respectively, in the PCR reaction. This method was proved to be suitable for simultaneous detection of two viruses with high specificity and sensitivity. The data showed that the method would allow the detection of about 100 viral particles in 1 mg of tissue. Furthermore, the amplification of viral DNA was not affected by the shrimp DNA.