Purified Canola Lutein Selectively Inhibits Specific Isoforms of Mammalian DNA Polymerases and Reduces Inflammatory Response |
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Authors: | Sho Horie Chiaki Okuda Takatoshi Yamashita Kenichi Watanabe Kouji Kuramochi Masashi Hosokawa Toshifumi Takeuchi Makiko Kakuda Kazuo Miyashita Fumio Sugawara Hiromi Yoshida Yoshiyuki Mizushina |
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Affiliation: | 1. Laboratory of Food and Nutritional Sciences, Department of Nutritional Science, Kobe-Gakuin University, Nishi-ku, Kobe, Hyogo, 651-2180, Japan 3. J-Oil Mills Inc., Tsurumi-ku, Yokohama, Kanagawa, 230-0053, Japan 4. Graduate School of Life and Environmental Science, Kyoto Prefectural University, Sakyo-ku, Kyoto, 606-8522, Japan 5. Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan 6. Department of Applied Biological Science, Tokyo University of Science, Noda, Chiba, 278-8510, Japan 2. Cooperative Research Center of Life Sciences, Kobe-Gakuin University, Chuo-ku, Kobe, Hyogo, 650-8586, Japan
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Abstract: | In the screening of DNA polymerase (pol) inhibitor, we isolated lutein, a carotenoid, from the crude (unrefined) pressed oil of canola (low erucic acid rapeseed, Brassica napus L.). Commercially prepared carotenoids such as lutein (1), zeaxanthin (2), β-cryptoxanthin (3), astaxanthin (4), canthaxanthin (5), β-carotene (6), lycopene (7), capsanthin (8), fucoxanthin (9) and fucoxanthinol (10), were investigated for the inhibitory activities of pols. Compounds 1, 2 and 8 exhibited strong inhibition of the activities of mammalian pols β and λ, which are DNA repair- and/or recombination-related pols. On the other hand, all carotenoids tested had no influence on the activity of a mammalian pol α, which is a DNA replicative pol. Lutein (1) was the strongest pol inhibitor of mammalian pols β and λ in the prepared ten carotenoids tested, but did not influence of the activities of mammalian pols α, γ, δ and ε. The tendency for pols β and λ inhibition by these carotenoids showed a positive correlation with the suppression of TPA (12-O-tetradecanoylphorbol-13-acetate)-induced inflammation. These results suggest that cold pressed unrefined canola/rapeseed oil, or other oils with high levels of lutein and other carotenoids, may be useful for their anti-inflammatory properties. |
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