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A histopathological study of the pulp of dogs’ teeth after induction of experimental pulp inflammation for different periods of time
Authors:A. SHAHRAVAN  J. GHODDUSI  B. ESLAMI  A.F. RATEGAR
Affiliation:1. Department of Endodontics, School of Dentistry and Oral and Dental Disease Research Center, Kerman University of Medical Sciences, Kerman, Iran;2. Department of Endodontics, School of Dentistry and Dental Research Center, Mashad University of Medical Sciences, Mashad, Iran;3. Department of Oral & Maxillofacial Pathology, School of Dentistry and Dental Research Center, Shaheeh Beheshti University of Medical Sciences, Mashhad, Iran
Abstract:The purpose of this study was to investigate the reaction of the pulp of dogs’ teeth after insertion of soft carious dentin from freshly extracted human teeth into the buccal cavities for short and longer periods of time. Forty‐seven mature lower and upper teeth were used in this study. On the middle of the buccal side of the teeth, 3 × 5 mm class ν cavities were prepared, soft carious dentin from freshly extracted human teeth was inserted into the floor of the cavity, and those were filled with glass ionomer. The dogs were killed after 7, 14, 28 and 47 days using vital perfusion techniques. Six‐micrometre sections were prepared and blindly evaluated by pathologist. An inflammatory reaction occurred in all of the samples. Moderate to severe inflammation were shown in all periods except in one in the 7‐day period. Insertion of soft carious dentin gathered from freshly extracted human teeth into class ν cavities produced in dogs’ teeth for 7 days is a reproducible process and could be used in study of pulp pathology.
Keywords:Histopathologic  dog teeth  experimentally  inflammatory reaction  pulp inflammation  soft carious dentin
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