| 薄层层析后生物显影检测茶多酚组分的抑菌效果 |
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| 引用本文： | 黄洋,姜建国,严媛,姚开.薄层层析后生物显影检测茶多酚组分的抑菌效果[J].现代食品科技,2003,19(1):30-31. |
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| 作者姓名： | 黄洋 姜建国 严媛 姚开 |
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| 作者单位： | 1. 华南理工大学食品与生物工程学院,广州,510640
2. 四川大学食品工程系,成都,610065 |
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| 摘 要： | 用薄板在异丁醇 :醋酸 :水 ( 4:1:5 )的展开体系中层析分离茶多酚 ,得到茶多酚组分的四个色点 ,即茶多酚的主要成分EGCG、ECG、ECG、EC ,根据各组分的Rf值和标准品作对照 ,鉴定各色点对应的茶多酚组分 ;将完成分离的薄板覆盖于加有溴甲酚紫指示剂的大肠杆菌培养基上培养 ,观察到抑菌圈出现 ,其抑菌圈大小为EGCG >ECG >EGC >EC。
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| 关 键 词： | 薄层层析 茶多酚 抑菌 |
| 修稿时间： | 2002年11月7日 |
Biography Detecting The Bacteriosyatic Effects of Compounds of Tea Polyphenols After Thin-Layer Chromatography |
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| Huanh Yang,Jianh Jian-guo,Yan Yuan,Yao Kai.Biography Detecting The Bacteriosyatic Effects of Compounds of Tea Polyphenols After Thin-Layer Chromatography[J].Modern Food Science & Technology,2003,19(1):30-31. |
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| Authors: | Huanh Yang Jianh Jian-guo Yan Yuan Yao Kai |
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| Affiliation: | Huanh Yang,1Jianh Jian-guo,1Yan Yuan,2Yao Kai |
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| Abstract: | Thin-layer chromatography whih solvent system of isobutyl alcohol,actic acid,and water with the ratio 4:1:5 was used to separate tea polyphenols.Four color spots were observed after the separation,which are the main components of tea polyphenols EGCG,EGC,ECG and EC.Each component was identified by its value of Rf and by contrast with the standard. The sheet performed separating was covered on the E.coli culture medium which was added indicator bromcresol purple,and bacteriostatic circles were obviously observed.The circles'relative scale list as EGCG>ECG>EGC>EC. |
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| Keywords: | thin-layer chromatography tea polyphenols bacteriostasis |
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