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Cryo-techniques applied to stratum corneum with description of a new sample holder for cryo-scanning electron microscopy of freeze-fractured samples |
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| Authors: | Hallegot,Minondo,& Fiat |
| Affiliation: | L'Oréal Recherche, Department of Analytical Chemistry, 1 av. Eugène Schueller, BP22, 93601 Aulnay-sous-Bois Cedex, France |
| Abstract: | Stratum corneum structure greatly differs from that of the living epidermis and specific sample cryo-preparation techniques have to be used. Practical aspects of these cryo-techniques applied to stratum corneum are discussed. Emphasis is placed on scanning electron microscopy of cryo-fixed samples. A new sample holder designed for cryo-scanning electron microscopy of freeze-fractured stratum corneum is described. |
| Keywords: | Cryofield emission scanning electron microscope (cryo-FESEM) cryo-scanning electron microscope (cryo-SEM) field emission scanning electron microscope (FESEM) freeze-fracture freeze substitution hexamethyldisilazane (HMDS) scanning electron microscope (SEM) stratum corneum |