Polysaccharide surface engineering of poly(D, L-lactic acid) via electrostatic self-assembly technique and its effects on osteoblast growth behaviours |
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Authors: | Kaiyong Cai Yuanliang Wang |
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Affiliation: | (1) College of Bioengineering, Key Lab of Biomechanics and Tissue Engineering of State Education Ministry, Chong Qing University, Chong Qing, 400044, P. R. China;(2) College of Bioengineering, Chong Qing University, Chong Qing, 400044, P. R. China |
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Abstract: | The objective of this study was to surface modify the poly (D, L-lactic acid) (PDLLA) films and assess the effects of the
modified surfaces on the functions of osteoblasts cultured in vitro. A layer-by-layer (LBL) self assembly technique, was used
leading to the formation of multilayers on the PDLLA film surfaces. Chitosan (Chi) and poly (styrene sulfonate, sodium salt)
(PSS) were utilized as polycation and polyanion in this study, respectively. The layer structure was investigated by using
X-ray photoelectron spectroscopy (XPS) and water contact angle measurement, respectively. XPS analysis displayed the presence
of chitosan on PDLLA surface. A full coverage of coating with PSS/Chi layers was achieved on the PDLLA surface only after
the deposition layers of PEI/(PSS/Chi)2. These results showed that PDLLA films could be modified with PSS/Chi pairs which may affect the biocompatibility of the
modified PDLLA films. To confirm this hypothesis, cell proliferation, cell viability as well as alkaline phosphtase activity
of osteoblasts on layer-by-layer modified PDLLA films as well as control samples were investigated in vitro. The proliferation
of osteoblasts on modified PDLLA films was found to be greater than that on control (p < 0.05 and p < 0.01) after 1, 4 and 7 days culture, respectively. Cell viability measurement showed that the PSS/Chi modified PDLLA films
have higher cell viability (p < 0.01) than control. Osteoblast differentiation function (ALP) on LBL-modified PDLLA film was found significantly higher
(p < 0.01) than that of virgin PDLLA films. These data suggests that PSS/Chi pair was successfully employed to surface modify
PDLLA film via a layer-by-layer technique, and enhanced its cell biocompatibility. |
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