| 碱性降解-Al~(3+)络合物形成荧光法测定强力霉素 |
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| 引用本文： | 赵一兵,王冬媛.碱性降解-Al~(3+)络合物形成荧光法测定强力霉素[J].福建分析测试,1996(2). |
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| 作者姓名： | 赵一兵 王冬媛 |
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| 作者单位： | 厦门大学化学系 厦门361005(赵一兵)，国家教委材料和生命过程分析科学开放研究实验室 厦门361005(王冬媛) |
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| 摘 要： | 本文提出了荧光法测定强力霉素(DC)的新方法。强力霉素碱性降解后与铝离子形成强荧光的络合物,并据此进行荧光测定,其荧光强度在4.0×10~(-8)～7.0×10~(-6)mol/L 范围内与强力霉素浓度成正比,共存的其它四环素类抗菌素不干扰强力霉素的测定,测定的检测限为3.0×10~(-8)mol/L。此法用于血清和尿液中强力霉素的测定,回收率分别为93～97%和91%～99%。
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| 关 键 词： | 碱性降解 荧光法 强力霉素 Al~(3+)络合物 |
Fluorimetric Determination of Doxycycline by Alkaline Degradation and Chelation with Al~(3+) |
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| Zhao Yibing Wang Dongyuan.Fluorimetric Determination of Doxycycline by Alkaline Degradation and Chelation with Al~(3+)[J].Fujian Anal Ysis & Testing,1996(2). |
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| Authors: | Zhao Yibing Wang Dongyuan |
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| Abstract: | A new fluorimetric method for the determination of trace doxycycline is proposed. The Alkaline degradation product of doxycycline(isodoxycycline)reacts rapidly with AI~(3+) and produces strong fluorescent chelate which is detected by fluorimetry. The fluorescence intensity of the chelate is proportional to the concentration of doxycyctine over the range of 4.0×10~(-8)～7.0×10~(-6)mol/L.Coexisting methacyctine and other tetracyclines do not inter- fere in the determination of doxycycline.The detection limits is 3.0×10~(-8)mol/L.This method has been used for the determination of doxycycline in serum and urine with 93%～ 97% and 91%～99% recoveries,respectively. |
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| Keywords: | Alkaline degeadation Fluorimetry Doxycycline Al~(3+)complex |
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