基于气相色谱-离子迁移谱技术鉴定内蒙古地区3个品种羊肉挥发性代谢物

分别选择放牧条件下6 只6 月龄体质量相近的乌珠穆沁羊、苏尼特羊和阿尔巴斯白绒山羊背最长肌为实验材料，采用气相色谱-离子迁移谱联用技术对3 个品种羊肉中的挥发性风味代谢物进行定性及定量测定，并对图谱差异进行分析，获得3 个品种羊肉的挥发性代谢物差异。运用主成分分析和偏最小二乘判别分析筛选乌珠穆沁羊肉与苏尼特羊肉、阿尔巴斯白绒山羊肉的差异挥发性代谢物。结果表明：在3 种羊肉中共检测到52 种挥发性风味代谢物，其中筛选并定性出乌珠穆沁羊肉与苏尼特羊肉差异代谢物7 种，分别为乙酸乙酯、丙酮、乙醇、2-丁酮、丁醛、壬醛和苯甲醛；乌珠穆沁羊肉与阿尔巴斯白绒山羊肉差异代谢物7 种，分别为丙酮、乙酸乙酯、2-丁酮、乙醇、苯甲醛、2-戊酮和3-羟基-2-丁酮；苏尼特羊肉与阿尔巴斯白绒山羊肉差异代谢物10 种，分别为丙酮、乙醇、2-丁酮、壬醛、乙酸乙酯、异戊醛、3-羟基-2-丁酮、2-戊酮、苯甲醛和辛醛。

Identification of Volatile Metabolites of Lame Meat from Three Ovine and Caprine Breeds in Inner Mongolia by Gas Chromatography-Ion Mobility Spectrometry

In this study, six grazing Ujimqin sheep, six grazing Sunit sheep and six grazing Albas white cashmere goats with similar body mass were selected and slaughtered at six months of age to collect Longissimus dorsi muscles. The volatile flavor metabolites in the meat samples were determined qualitatively and quantitatively by gas chromatography-ion mobility spectrometry (GC-IMS), and differential volatile metabolites between the three breeds were identified by principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). The results showed that 52 volatile flavor metabolites were detected in all samples, among which seven differential metabolites between Ujimqin and Sunit sheep meat were identified, namely ethyl acetate, acetone, ethanol, 2-butanone, butyraldehyde, nonanal and benzaldehyde; seven differential metabolites between Ujimqin sheep and Albas white cashmere goat meat, namely acetone, ethyl acetate, 2-butanone, ethanol, benzaldehyde, 2-pentanone and 3-hydroxy-2-butanone; and ten differential metabolites between Sunit sheep and Albas white cashmere goat meat, namely acetone, ethanol, 2-butanone, nonanal, ethyl acetate, isovaleraldehyde, 3-hydroxy-2-butanone, 2-pentanone, benzaldehyde and octanal.