葛仙米藻胆蛋白的体外消化特性

研究葛仙米藻胆蛋白在体外模拟胃液和肠液的消化产物性质，重点测定不同消化时间产物的可溶性蛋白质、游离氨基含量变化及其抗氧化作用，并利用HPLC法测定消化产物分子质量变化情况。结果表明：在胃液和肠液消化作用下蛋白质含量均呈现下降趋势，而游离氨基含量呈现上升趋势，消化产物较初始产物对ABTS+·和脂质过氧化具有更强的抑制作用，但对·OH的清除能力却有所下降；偏相关分析结果表明，与葛仙米藻胆蛋白消化产物的抗氧化作用紧密相关的是消化产物中的分子质量为6ku和0.2ku的肽类。

Digestion Characteristics in vitro of Phycobiliprotein from Nostoc sphaeroides Ku..ting

In this study,some properties of products produced during digestion of Nostoc sphaeroides ku..ting phycobiliprotein in simulated gastric and intestinal fluids were investigated especially such as soluble protein and free amino acid contents and antioxidant properties.Meanwhile,the change in molecular weight during digestion of Nostoc sphaeroides ku..ting phycobiliprotein was determined by HPLC.The results showed that protein content revealed a downward trend during digestion in each stimulated fluids,and consequently free amino acid content exhibited an upward trend.Digestion products had stronger ABTS radical scavenging and anti-lipid peroxidation activities but weaker hydroxyl radical scavenging activity in D-deoxyribose system than Nostoc sphaeroides ku..ting phycobiliprotein.The results of partial correlation analysis demonstrated that 6 ku and 0.2 ku peptides were closely related to the antioxidant effect of digestion products of Nostoc sphaeroides ku..ting phycobiliprotein.