The rat epididymal fat pad as an implantation site for the study of microcapsule biocompatibility: validation of the method |
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Authors: | JF Pariseau FA Leblond F Harel Y Lepage JP Hallé |
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Affiliation: | Centre de Recherche Guy Bernier, H?pital Maisonneuve-Rosemont, Montréal, Québec, Canada. |
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Abstract: | The study of microcapsule biocompatibility is hindered by their uneven distribution and low recovery when implanted into the peritoneum. We evaluated the use of the rat epididymal fat pad as a microcapsule implantation site for biocompatibility studies. The recovery rate of microcapsules containing 85Sr-labeled microspheres was 99.6 +/- 0.75%. Microcapsules made from the same batch of nonpurified alginate, were injected into both fat pads of male Lewis rats (n = 18) and retrieved 14 days later. A semiquantitative fibrosis score scaled from 0 to 3.0 showed that the pericapsular reaction was uniform throughout a fat pad, and that the results of the two fat pads were equivalent because the null hypothesis of inequivalence was rejected (P < .001). Thus, this method can be used to compare the biocompatibility of microcapsule of differing compositions. |
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