The effect of osmotic dehydrofreezing on the role of the cell membrane in carrot texture softening after freeze-thawing

To understand the protective mechanism of the osmotic dehydrofreezing technique on carrot texture after freeze-thawing, two mechanical texture parameters, fracture stress related to the cell wall and initial modulus related to the cell membrane, as well as cell membrane water permeability using PFG-NMR were evaluated. In particular, to understand the role of the cell membrane in texture alteration, tissue in which the cell membrane was exposed to chloroform vapor was used. Although dehydrofreezing protected texture from freezing damage, the effect was only observed with respect to fracture stress, with exhibited values close to those for raw tissue. However, there was no protective effect on initial modulus and water permeability, in which values did not differ from those of cell membrane-free tissue. More specifically, osmotic dehydrofreezing had no effect on the cell membrane induced by freeze-thawing.