A process for the synthesis of PUFA-enriched triglycerides from high-acid crude fish oil |
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Authors: | Weifei Wang Tie Li Zhengxiang NingYonghua Wang Bo Yang Yongjun MaXiaoquan Yang |
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Affiliation: | a College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, People’s Republic of China b School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, People’s Republic of China c Zhejiang Industrial Group Co. Ltd., Zhoushan 316101, People’s Republic of China |
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Abstract: | This paper reports a research interest, that aims to establish a feasible industrial process, for the enzymatic production of glycerides with highly concentrated n-3 polyunsaturated fatty acids (eicosapentaenoic acid and docosahexaenoic acid), from high-acid crude fish oils. The process covers enzymatic deacidification of crude fish oil, alkaline-based synthesis of polyunsaturated fatty acids ethyl esters, concentration of polyunsaturated fatty acids ethyl esters, and enzyme catalyzed synthesis of polyunsaturated fatty acids glycerides. Commercial available immobilized lipase, Novozym 435, was used as the catalyst. The synthesized glycerides contained 5.5% of eicosapentaenoic acid and 74.6% of docosahexaenoic acid, which was 1.21 and 2.71 times more than that in the original fish oil, respectively. The application of the designed process was successfully proved by a 100-fold scale-up reaction. |
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Keywords: | Eicosapentaenoic acid Docosahexaenoic acid High-acid fish oil Deacidification Transesterification Glycerolysis |
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