Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells |
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Authors: | M López-Álvarez E L Solla P González J Serra B León A P Marques R L Reis |
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Affiliation: | (1) Department of Applied Physics, E.T.S.I. Industriales, University of Vigo, Campus Lagoas-Marcosende, 36310 Vigo, Spain;(2) 3B’s Research Group—Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal;(3) IBB—Institute for Biotechnology and Bioengineering, Braga, Portugal |
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Abstract: | The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over
the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite
were respectively the silicon and HA sources used to produce the Si–HA coatings. HA coatings with 0 wt% of silicon were used
as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si–HA
thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of
Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic
activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis
demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic
morphology along the time of culture. The Si–HA coatings did not evidence any type of cytotoxic behaviour when compared with
HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance
on the Si–HA coatings from diatoms than on the Si–HA from silica. |
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