A simple regression method for determining the quantity of translated protein in a cell-free protein synthesis system |
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Authors: | Kang T J Kang S H Jung S T Lee E Y Choi C Y |
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Affiliation: | Department of Chemical Technology, College of Engineering, Seoul National University, Seoul 151-742, Korea. |
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Abstract: | A simple regression method for quantifying endogenous leucine in S30 extract was developed. This method enabled the quantity of translated protein in a cell-free protein synthesis system to be exactly determined. |
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