| 氡染毒大鼠支气管肺泡灌洗液蛋白质的双向电泳分析 |
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| 引用本文： | 徐乃玉,张素萍,聂继华,丁建松,童建.氡染毒大鼠支气管肺泡灌洗液蛋白质的双向电泳分析[J].辐射研究与辐射工艺学报,2007,25(3):171-174. |
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| 作者姓名： | 徐乃玉 张素萍 聂继华 丁建松 童建 |
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| 作者单位： | 1. 苏州大学药学院,苏州,215123
2. 苏州大学放射医学与公共卫生学院,江苏省放射医学与防护重点实验室,苏州,215123 |
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| 基金项目： | 国家自然科学基金
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江苏省研究生培养创新工程项目 |
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| 摘 要： | 观察氡染毒后大鼠支气管肺泡灌洗液(Bronchoalveolar lavage fluid,BALF)蛋白质表达谱的变化.雄性Wistar大鼠吸入氡的累积剂量达200工作水平月(Working level month,WLM)后,收集大鼠BALF,提取BALF细胞总蛋白,采用双向电泳技术(Two-dimensional electrophoresis,2-DE)分离总蛋白质,银染显色,进行差异蛋白质的分析.ImageMaster 2D Platinum软件分析结果显示正常组和氡染毒组大鼠BALF细胞总蛋白双向电泳图谱上分别可见到645和554个蛋白点,共获得48个显著的蛋白质差异点,其中23个点在正常大鼠BALF中表达或表达明显增强,25个点在染毒大鼠BALF中表达或表达明显增强.结果提示,正常大鼠与氡染毒大鼠BALF细胞的蛋白质表达谱存在差异.
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| 关 键 词： | 双向电泳 氡 肺泡灌洗液 蛋白质组 |
| 修稿时间： | 2006-12-042007-02-07 |
Analysis of different expression proteins of Bronchoalveolar lavage fluid in rats radon-inhaled by two-dimensional gel electrophoresis |
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| XU Naiyu,ZHANG Suping,NIE Jihua,DING Jiansong,TONG Jian.Analysis of different expression proteins of Bronchoalveolar lavage fluid in rats radon-inhaled by two-dimensional gel electrophoresis[J].Journal of Radiation Research and Radiation Processing,2007,25(3):171-174. |
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| Authors: | XU Naiyu ZHANG Suping NIE Jihua DING Jiansong TONG Jian |
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| Affiliation: | Department of Pharmacy, Soochow University, Suzhou 215123 ;School of Radiation Medicine and Public Health, Soochow University, The Key Laboratory of Radiation Medicine and Protection of Jiangsu Province, Suzhou 215123 |
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| Abstract: | In this paper the different protein expression of BALF in rats inhaled radon was analyzed by use of two-dimensional electrophoresis (2-DE). Male Wistar rats were exposed to radon with the cumulative dose up to 200 working level month (WLM), then the bronchoalveolar lavage fluid (BALF) was collected and the total proteins were extracted. 2-DE was employed to isolate the total proteins of BALF. The gels were stained with silver and differential protein expressions were analyzed with Image Master 2D Platinum software. The total protein spots in the gels of the control and radon-inhaled rats were 654 and 554 respectively, containing 48 differential protein spots in which 23 ex-pressed and obviously increased in the control group and 25 expressed and obviously increased in rats radon-inhaled group. The results indicate that the protein expression of BALF between rats radon-inhaled and the control are dif-ferent to some extent. |
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| Keywords: | Two-dimensional electrophoresis (2-DE) Radon Bronchoalveolar lavage fluid (BALF) Proteome |
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