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Behavioral Response of Males to Major Sex Pheromone Component, (Z,Z)-5,25-Hentriacontadiene, of Drosophila ananassae Females
Authors:Motomichi Doi  Tadashi Nemoto  Hiroshi Nakanishi  Yasumasa Kuwahara  Yuzuru Oguma
Affiliation:1. Institute of Biological Sciences, University of Tsukuba, Tsukuba, 305, Japan
2. Biomolecules Department, National Institute of Bioscience and Human-Technology, Tsukuba, 305, Japan
3. Pesticide Research Institute, Faculty of Agriculture, Kyoto University, Kyoto, 606-01, Japan
Abstract:To identify sex pheromone components in Drosophila ananassae, the cuticular hydrocarbons were analyzed and bioassayed. After silicic acid and two silver nitrate–silicic acid chromatographic steps, active fractions containing (Z,Z)-5,25- and (Z,Z)-4,26-hentriacontadiene as the most abundant components were obtained. Both hydrocarbons could elicit male courtship behavior. The former was the more predominant compound and elicited a higher courtship index value than the latter. Cuticular hydrocarbon differences between D. ananassae and its close relative, D. pallidosa, and their roles in species recognition are discussed.
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