源于 GAP 启动子的毕赤酵母组成型表达系统的研究进展

巴斯德毕赤酵母表达系统是目前应用非常广泛的外源基因真核表达系统。与源于醇氧化酶启动子(pAOX1)的诱导型表达系统相比, 源于三磷酸甘油醛脱氢酶启动子(pGAP)的毕赤酵母组成型表达系统在表达外源蛋白时不需使用甲醇, 表达时间短, 并且在高密度发酵时采用连续发酵模式, 因此成为近年研究的热点。本文分别从pGAP表达载体的构建、碳源、基因拷贝数、信号序列、高密度发酵等方面对该表达系统进行了综述, 以期为其在蛋白生产中的深入研究和应用提供参考。

Progress in GAP promoter derived constitutive expression system of Pichia pastoris

The Pichia pastoris expression system, which is one of the eukaryotic expression systems for heterologous gene expression, is widely used at present. Compared with the alcohol oxidase I promoter (pAOX1) derived inductive expression system, the glyceraldehyde-3-phosphate dehydrogenase promoter(pGAP) derived constitutive expression system of Pichia pastoris avoids the use of methanol, needs shorter period, and adopts continuous fermentation mode, thus it becomes a hot point in recent researches. The construction of pGAP expression vectors, carbon sources, gene copy number, signal sequence, and high-density cell culture of this expression system were summarized in this paper to provide references for further study and application of this expression system in protein expression fields.