酶解辅助水法提取土茯苓总黄酮工艺条件研究

采用了酶解辅助水法提取土茯苓总黄酮．以芦丁为标准品，用铝离子显色、紫外-可见分光光度法测定总黄酮含量．以总黄酮提取率为考察指标，通过单因素实验探讨酶用量、固液比、提取温度、提取次数对总黄酮提取率的影响，优化提取条件．结果表明最佳提取工艺条件为：酶用量2．0％、固液比例1：8、提取温度100℃、提取3次最佳条件下，粗提物干粉得率为14．0％，干粉总黄酮含量为4．1％，总黄酮提取率为0．57％．

Study on the Extraction Process of Total Flavonoids from Smilax Glabra by Enzyme-assisted Hydrolysis

The total flavonoids was Extracted from Smilax glabra by a enzyme-assisted hydrothermal method; Using Rutin as standard, the content of the total flavonoids was measured by ultraviolet and visible spectrophotometry with aluminum ion colour. The extraction ratio of total flavonoids was investigated as a main target. The effects of amount of enzyme, the solid-liquid ratio, extraction temperature and extraction times on the total extraction ratio of flavonoids were discussed by means of the single-factor experiment to optimize the extraction conditions. The results showed that： for the best extraction conditions the enzyme dosage is 2.0% with the solid-liquid ratio being 1：8 and extraction frequency being 3 times at 100 ℃. In the best conditions, the powder ratio of crude extraction is 14.0%, and the content of total flavonoids in this powder is up to 4.1% with the extract ratio of total flavonoids being 0.57%.