| 桦褐孔菌多糖测定方法的比较 |
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| 引用本文： | 孟玲,王兰英,梁大勇,王丽华,王丽华,宋爱荣.桦褐孔菌多糖测定方法的比较[J].食品研究与开发,2010,31(4). |
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| 作者姓名： | 孟玲 王兰英 梁大勇 王丽华 王丽华 宋爱荣 |
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| 作者单位： | 山东省应用真菌重点实验室,青岛农业大学药用真菌研究所,山东,青岛,266109 |
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| 摘 要： | 为选出一种比较准确、方便的测定真菌多糖的方法,以桦褐孔茵茵丝体发酵液提取的粗多糖为原料,对苯酚一硫酸法、蒽酮-比色法、斐林滴定法3种方法进行比较.3种方法比较而言,苯酚-硫酸法与蒽酮-比色法测得的糖含量相差甚微,分别为61.07%、62.25%.苯酚-硫酸法显色不稳定,蒽酮-比色法比较容易操作,测定的多糖含量和回收率均最高,回收率为125%;斐林滴定法回收率较低45%,测得的多糖含量偏低为33.12%,最终确定蒽酮-比色法为最佳测定方法.
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| 关 键 词： | 桦褐孔菌 苯酚-硫酸法 蒽酮-比色法 斐林滴定法 多糖含量 |
The Comparison of Polysaccharide Determination Methods about Phaeoporus Obliquus |
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| MENG Ling,WANG Lan-ying,LIANG Da-yong,WANG Li-hua,WANG Li-hua,SONG Ai-long.The Comparison of Polysaccharide Determination Methods about Phaeoporus Obliquus[J].Food Research and Developent,2010,31(4). |
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| Authors: | MENG Ling WANG Lan-ying LIANG Da-yong WANG Li-hua WANG Li-hua SONG Ai-long |
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| Affiliation: | MENG Ling,WANG Lan-ying,LIANG Da-yong,WANG Li-hua,SONG Ai-rong* (The Institute of Medical Fungi Qingdao Agriculture University,Laboratory of Applied Mycology of Sh,ong Province,Qingdao 266109,Sh,ong,China) |
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| Abstract: | In order to select an accurate, convenient method of fungi polysaccharide determination, the methods of Anthrone-sulfuric method, phenol-sulfuric method and direct-titration method were compared. The results showed: Among the three methods, there were not many differences between Phenol-sulfuric method and Anthrone-sulfuric method,contents of the polysaccharide were 61.07 %,62.25 %. Phenol-sulfuric method showed color development was not very steady while Anthrone-sulfuric method was easier to operate. Anth... |
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| Keywords: | Phaeoporus obliquus phenol-sulfuric method anthrone-sulfuric method direct-titration method polysaccharide content |
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