芳樟树叶乙醇提取物的抗癌作用

探讨了芳樟树叶乙醇提取物各部分的抗癌作用。采用系统溶剂法从芳樟树叶得到一系列提取物，运用MTT和集落形成实验法考察各提取物对体外培养的人肺癌95-D 细胞、人口腔表皮样癌KB 细胞和肝癌HepG2细胞增殖的影响。结果发现石油醚部分A1、氯仿部分A2和乙酸乙酯部分A3对3种肿瘤细胞的增殖和集落形成均有不同程度的抑制作用，且给药量与细胞抑制和集落形成明显的正相关关系。因此，芳樟树叶乙醇提取物在体外有明显的抗肿瘤作用，且抗癌有效组分很可能在它的石油醚部分、氯仿部分以及乙酸乙酯部分。

Antitumor action of ethanolic extractives from camphor leaves

The in vitro antitumor action of the different parts of ethanolic extractives from Camphor leaves，which were obtained by the technique of solvent-refining，was investigated in this paper. The dried Camphor leaves were submitted to ethanol（95%v/v）extraction and then the ethanolic extract was extracted again by petrol-ether，chloroform，ethyl acetate，methanol and water successively，and five extractives - A1，A2，A3，A4 and A5，were attained correspondingly. Using MTT and colony-forming methods，the five extractives were tested for the cytotoxicity on high metastatic lung carcinoma cells (95-D)，human oral epidermoid carcinoma (KB) and the human hepatoma cell line (HepG2). The extrac- A1，A2 and A3，showed the significant antitumor action when compared with the controls. The three extractives inhibited the tumor cells viability in concentration–dependent characteristics. These investigations suggested that the extract from camphor leaves had the function of antitumor in vitro，which was reported here firstly.