| 酿酒酵母与粟酒裂殖酵母融合子检出方法的研究 |
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| 引用本文： | 袁耀武,张伟,程书梅,苏旭东,何义. 酿酒酵母与粟酒裂殖酵母融合子检出方法的研究[J]. 中国酿造, 2008, 0(23) |
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| 作者姓名： | 袁耀武 张伟 程书梅 苏旭东 何义 |
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| 作者单位： | 河北农业大学食品科技学院; |
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| 基金项目： | 河北省“十五”重大科技攻关专项(03220171D) |
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| 摘 要： | 为了快速检出酿酒酵母与粟酒裂殖酵母的融合子,在双亲原生质体融合处理后,利用两亲本再生营养条件的差异及氧化邻苯二胺能力的不同,通过设计选择性底层培养基和鉴定性上层培养基,进行了融合子检出方法的研究,并确定了3株融合子。该方法简单易行,可用于酿酒酵母与粟酒裂殖酵母原生质体融合育种的研究。
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| 关 键 词： | 酿酒酵母 粟酒裂殖酵母 原生质体融合 融合子 |
Research on detection method of the fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe |
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| YUAN Yaowu,ZHANG Wei,CHENG Shumei,SU Xudong,HE Yi. Research on detection method of the fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe[J]. China Brewing, 2008, 0(23) |
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| Authors: | YUAN Yaowu ZHANG Wei CHENG Shumei SU Xudong HE Yi |
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| Affiliation: | YUAN Yaowu,ZHANG Wei,CHENG Shumei,SU Xudong,HE Yi (Department of Food Science , Technology,Hebei Agricultural University,Baoding 071001,China) |
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| Abstract: | In order to select the fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe speedily, the detection method for fusant was designed using selective bottom medium and identification upper medium according to the different nutritious requirements for regeneration and dif-ferent o-phenylenediamine oxidizing abilities of the parent strains after protoplast fusion. With above method, three fusants were obtained. The method was simple, easy, and could be used to the breeding of S. cerevisiae and S. p... |
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| Keywords: | Saccharomyces cerevisiae Schizosaccharomyces pombe protoplast fusion fusant |
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