儿茶酚–(胺)表面化学介导的一氧化氮催化释放涂层研究

目的 在316L不锈钢(SS)表面构建原位一氧化氮(NO)催化释放涂层,使其能特异选择性地抗凝血、抑制平滑肌细胞(SMCs)增生,从而促进内皮细胞(ECs)生长。方法 在弱碱性水溶液中,选用多巴胺(DA)和己二胺(HD)为前驱体,利用多巴胺邻二苯酚结构自聚合沉膜的能力、多巴胺与己二胺的酚–(胺)表面化学,通过简单的一锅法在316L SS表面构建富氨基粘附涂层DA/HD。再通过碳二亚胺化学交联反应,共价接枝螯合Cu²⁺的1,4,7,10–四氮杂环十二烷–1,4,7,10–四羧酸(DOTA),最后获得均匀且稳定的NO催化释放涂层(命名为Cu-DOTA@DA/HD)。结果 DA/HD涂层表面的氨基密度高达22 nmol/cm²,实现了Cu-DOTA的有效固定,其NO催化释放速率可达5.2×10^–10 mol/(cm²·min)。Cu-DOTA@DA/HD涂层显著地抑制了血小板的粘附和激活,也能有效抑制血栓的形成,其表面血栓总质量由316L SS的(40.3±10.3) mg降低至(3.0±0.4) mg。...

Chemical-mediated Nitric Oxide-generating Coatings on Catechol-(Amine) Surface

It is an advanced surface technology that can improve the biocompatibility and hemocompatibility of blood contact-like material surfaces by constructing an in situ nitric oxide (NO) catalytic release coating on the surface of 316L stainless steel (SS) without changing the physical properties of the substrate material. The biocompatibility and hemocompatibility of unmodified 316L stainless steel (SS) are too poor to cause complications such as acute thrombosis and smooth muscle cell proliferation after implantation, which can inhibit the regenerative repair of diseased tissue. Thus, in this work, a NO-catalyzed coating based on the chemical construction of catechol-(amine) surface was investigated, and a sustained and stable NO-catalyzed release from the surface of 316L SS was successfully achieved, endowing it with multiple biological functions such as anti-coagulation, selective inhibition of SMCs proliferation and promotion of EC growth.