| 几丁聚糖提取方法的改进及SAS应用 |
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| 引用本文： | 杨铭铎,刘浩宇,王禾,李元瑞.几丁聚糖提取方法的改进及SAS应用[J].食品与发酵工业,2001,27(9):42-46. |
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| 作者姓名： | 杨铭铎 刘浩宇 王禾 李元瑞 |
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| 作者单位： | 1. 黑龙江商学院中式快餐研究发展中心,
2. 西北农林科技大学食品科学与工程学院, |
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| 基金项目： | 国家人事部留学归国人员科技活动资助项目(人200106AD) |
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| 摘 要： | 利用酶与酸碱相结合 ,从废弃虾蟹壳中提取几丁聚糖 ,与传统方法相比 ,具有降低成本 ,减少污染等优点 ;通过SAS系统软件得到最佳工艺参数。最佳酶解条件为 :体积比 1∶16,加酶量 140 0 0IU/ g ,pH7.0 ,温度 5 5℃ ,反应时间 5h ;脱乙酰基条件为温度 110℃ ,碱浓度4 6% ,反应时间 2h ;通过测定几丁聚糖的主要质量指标脱乙酰度和粘度 ,证明了该工艺的可行性
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| 关 键 词： | 几丁聚糖 木瓜蛋白酶 脱乙酰度 SAS系统软件 |
| 修稿时间： | 2001年4月28日 |
Improved Extraction Method for Chitosan and Application of SAS System in this Process |
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| Yang Mingduo , Liu Haoyu Wang He Li Yuanrui.Improved Extraction Method for Chitosan and Application of SAS System in this Process[J].Food and Fermentation Industries,2001,27(9):42-46. |
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| Authors: | Yang Mingduo Liu Haoyu Wang He Li Yuanrui |
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| Affiliation: | Yang Mingduo 1,2 Liu Haoyu 1 Wang He 1 Li Yuanrui 2 1 |
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| Abstract: | Chitosan was preparated from shrimp and crab shells by combination of enzyme with acid and alkali method. By comparing with the traditional method, it has saved money and protected environment The best process was got by SAS That is, when papain acted on the materials, the best proportion was water:papain=16:1,pH is 7 0, under the condition of 55℃,14?000?IU/g, for 5 hours Deacetylation was achieved by 46% NaOH at 110℃ for 2 hours By determinating deacetylation degree and viscosity, the most important evaluation factors for chitosan, this technology was proved to be feasibility. |
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| Keywords: | chitosan papain deacetylation SAS system |
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