Separation of perillyl alcohol from the peel of citrus unshiu by supercritical CO2 and preparative high-performance liquid chromatography |
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Authors: | Chong Ho Lee Youn-Woo Lee Jae-Duck Kim Kyung Ho Row |
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Affiliation: | (1) Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University, 402-751 Incheon, Korea;(2) KIST, Environment Remediation Research Center, 136-791 Seoul, Korea |
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Abstract: | To separate perillyl alcohol (POH), a potential anti-cancer agent, the peel of citrus unshiu was extracted by supercritical
CO2 extraction (SCE) system at 50 ‡C, 200 bar and 6 kg CO2/hr/kg sample. The extracts were partitioned by acetonitrile/hexane (90/10, vol%). POH was eluted in the acetonitrile phase.
An open-tubular chromatography with silica gel (40–63 Μm) was used to purify POH from the acetonitrile phase. Mobile phase
was hexane/ethyl acetate (90/10, vol%). To obtain POH in a pure form, finally preparative high-performance liquid chromatography
was applied. The collection of POH from citrus unshiu peel was achieved on a laboratory-prepared Chromatographic column (300x3.9
mm) packed with 15 Μm C18 preparative packings. The composition of mobile phase was water/acetonitrile (50/50, vol%). The flow rate of the mobile phase
was 1 ml/min and UV wavelength was fixed at 205 nm. It was found that the total yield of POH was 1.6xl0-3 (wt%) as the dry powder of citrus unshiu peel. |
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Keywords: | Perillyl Alcohol Citrus Unshiu Peel Supercritical Fluid Extraction Preparative HPLC |
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