LC-MS/MS Determination of Organophosphorus Pesticide Residues in Coconut Water |
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Authors: | Pragney Deme Tirupathi Azmeera Sanjit Kanjilal Padmaja Jonnalagadda Vijaya Saradhi Upadhyayula |
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Affiliation: | 1. National Centre for Mass Spectrometry, Indian Institute of Chemical Technology, Taranaka, Hyderabad, Andhra Pradesh, India, 500007 2. Lipid Science and Technology Division, Indian Institute of Chemical Technology, Taranaka, Hyderabad, Andhra Pradesh, India, 500007 3. Food and Drug Toxicology Research Center, National Institute of Nutrition, Taranaka, Hyderabad, Andhra Pradesh, India, 500007
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Abstract: | A simple and specific analytical method based on solid phase extraction (SPE), and liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS) operated in multipule reaction monitoring (MRM) mode with dynamic time segment acquisition windows, was developed to determine organophosphorus pesticide residues (acephate, monocrotophos, dimethoate, malaoxon, dichlorvos, malathion, phenthoate, parathion-ethyl, chlorfenvinfos, quinalphos, diazinon, phosalone, profenofos, ethion, chlorpyrifos) in tender coconut water. The method was observed to be linear with lower limit of detections ranged from 0.1 to 1.5 ng/mL (measured at S/N ratio 5:1) and limit of qunatitation 0.5 to 2.0 ng/mL (measured at S/N ratio 10:1) for all studied pesticides. The intra- and inter-day assay accuracies calculated at four concentration levels in six replicates (n?=?6) were in the range of 80 % to 115 % with relative standard deviations (RSD) below 15 %. The percentage recoveries of all the analysed pesticides were in the range of 86.8 % to 107.6 % and the calculated RSD values were below 12 %. The developed method was applied to determine pesticide residues in natural tender coconut water samples. The results showed the presence of malathion ranging from 24 to 45 ng/L concentrations in some natural tender coconut water samples collected from various local market places in and around Hyderabad, Andhra Pradesh, India. |
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