Uptake of fertilizer and soil nitrogen by ryegrass swards during spring and mid-season

Double-labelled¹⁵N ammonium nitrate was used to determine the uptake of fertilizer and soil N by ryegrass swards during spring and mid-season. The effects of water stress (40% of mean rainfall v 25 mm irrigation per 25 mm soil water deficit) and the rate of application of N in the spring (40 v 130 kg ha^–1) on the recovery of 130 kg N ha^–1 applied in mid-season were also evaluated. Apparent recovery of fertilizer N (uptake of N in the fertilized plot minus that in the control expressed as a percentage of the N applied) was 95 and 79% for fertilizer N applied in the spring at rates of 40 and 130 kg ha^–1, respectively. Actual recovery of the fertilizer N assessed from the uptake of¹⁵N was only 31 and 48%, respectively. The uptake of soil N by the fertilized swards was substantially greater than that by the control. However, the increased uptake of soil N was always less than the amount of fertilizer N retained in or lost from the soil. Broadly similar patterns for the uptake of fertilizer and soil N were observed during mid-season. Uptake of N in mid-season was highest for swards which received 40 kg N ha^–1 in the spring and suffered minimal water stress during this period. Application of 130 kg N ha^–1 in spring reduced the uptake of N in mid-season to an extent similar to that arising from water stress. Only 1.8 to 4.2 kg ha^–1 (3 to 10%) of the N residual from fertilizer applied in the spring was recovered during mid-season. Laboratory incubation studies suggested that only a small part of the increased uptake of soil N by fertilized swards could be attributed to increased mineralisation of soil N induced by addition of fertilizer. It is considered that the increased uptake of soil N is partly real but mostly apparent, the latter arising from microbially mediated exchange of inorganic¹⁵N in the soil.