Comparison of analyses of surfactants in cosmetics using high-performance liquid chromatography and high-performance capillary electrophoresis |
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Authors: | Wen-Chung Lin Shaw-Tao Lin Shwu-Ling Shu |
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Affiliation: | (1) Department of Applied Chemistry, Providence University, Sha-Lu, 43301 Taichung Hsien, Taiwan, Republic of China |
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Abstract: | Separation of anionic, cationic, and amphoteric surfactants containing n-dodecyl groups and hydrophilic moieties was done by high-performance liquid chromatography (HPLC) and high-performance capillary
electrophoresis (HPCE), and an ultraviolet-visible detector. Quantitation of surfactants in commercial cosmetic and toiletry
products was also done using similar methods. Conditions used to separate mixtures of surfactants by HPLC are as follows:
stationary phase: ODS 2; mobile phase: MeOH/H2O (80∶20, vol/vol) containing 1.0M NH4Cl, 0.003 M tetrabutylammonium hydrogen sulfate, and 0.005 M diammonium phosphate buffer solution at pH=6.0. Conditions for
HPCE are as follows: an uncoated capillary (100 μm i.d., 110 cm in length, effective length of 75 cm) with 25 kV of applied
voltage, and an aqueous buffer containing 80 mM sodium borate/20 mM NaOH at pH=9.2. Surfactants were eluted within 15 min.
The accuracy of both techniques was evaluated by analyzing the recovery ratio of surfactants in the mixture. |
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Keywords: | Critical micelle concentration electroosmotic flow electrophoretic separation high-performance capillary electrophoresis high-performance liquid chromatography ion-paired reagents surfactants UV detectors |
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