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The Peculiar Structure of Acetobacter pasteurianus CIP103108 LPS Core Oligosaccharide |
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| Authors: | Prof. Roberta Marchetti Ferran Nieto Fabregat Dr. Mateusz Pallach Dr. Djamel Gully Dr. Eric Giraud Prof. Antonio Molinaro Dr. Katarzyna A. Duda Alba Silipo |
| Affiliation: | 1. Department of Chemical Sciences, University of Naples Federico II Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126 Napoli, Italy;2. Department of Chemical Sciences, University of Naples Federico II Complesso Universitario Monte Sant'Angelo, Via Cintia 4, 80126 Napoli, Italy Present address: Department of Organic Chemistry, Stockholm University, Svante Arrhenius väg 16C, 106 91 Stockholm, Sweden;3. IRD, Laboratoire des Symbioses Tropicales et Méditerranéennes (LSTM) UMR IRD/SupAgro/INRA/UM2/CIRAD, TA-A82/J, Campus de Baillarguet, 34398 Montpellier Cedex 5, France;4. Junior Group of Allergobiochemistry, Research Center Borstel Leibniz Lung Center, Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany Airway Research Center North (ARCN), German Center for Lung Research, 23845 Borstel, Germany |
| Abstract: | Acetobacter pasteurianus, a member of the Alphaproteobacteria, is an acetic acid-producing bacterium present on sugar-rich substrates such as such as fruits, flowers and vegetables and traditionally used in the production of fermented food. The preferred living habitat associated with acid conditions makes the structure of the bacterial cell wall interesting to study, due to expected uncommon features. We have used a combination of chemical, analytical and NMR spectroscopy approaches to define the complete structure of the core oligosaccharide from A. pasteurianus CIP103108 LPS. Interestingly, the core oligosaccharide displays a high concentration of negatively charged groups, structural features that might contribute to reinforcing the bacterial membrane. |
| Keywords: | acetic acid bacteria Acetobacter pasteurianus core oligosaccharide lipopolysaccharide NMR spectroscopy |