| Affiliation: | 1. Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV) Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Irapuato, Guanajuato, 36824 México Centro de Investigación y de Estudios Avanzados del IPN Unidad Irapuato, Departamento de Ingeniería Genética, Irapuato, Guanajuato, 36824 México;2. Centro de Investigación y de Estudios Avanzados del IPN Unidad Irapuato, Departamento de Ingeniería Genética, Irapuato, Guanajuato, 36824 México;3. CONACYT, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV) Unidad de Genómica Avanzada, Laboratorio Nacional de Genómica para la Biodiversidad (Langebio), Irapuato, Guanajuato, 36824 México |
| Abstract: | Cysteine-rich peptides (CRPs) are small proteins of less than 100 amino acids in length characterized by the presence of disulfide bridges and common end-to-end macrocyclization. These properties confer hyperstability against high temperatures, salt concentration, serum presence, and protease degradation to CRPs. Moreover, their intercysteine domains (loops) are susceptible to residue hypervariability. CRPs have been successfully applied as stable scaffolds for molecular grafting, a protein engineering process in which cysteine-rich structures provide higher thermodynamic and metabolic stability to an epitope and acquire new biological function(s). This review describes the successes and limitations of seven cysteine-rich scaffolds, their bioactive epitopes, and the resulting grafted peptides. |
