Surface Ligand Density Switches Glycovesicles between Monomeric and Multimeric Lectin Recognition |
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Authors: | Shivender Yadav Dr Kottari Naresh Prof Dr Narayanaswamy Jayaraman |
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Affiliation: | 1. Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560012 India;2. Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560012 India
Present address: HP Green R&D Centre, KIADB Industrial Area, Bangalore, 560 067 India |
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Abstract: | Carbohydrate-protein interactions define a multitude of cellular recognition events. We present herein synthetic glycovesicles as cell-surface mimics in order to switch the nature of lectin recognition. The covalent glycovesicles, constituted with diacetylene monomers of various ligand densities at their surfaces, are prepared through photo-polymerization. Vesicles with sparsely imbedded ligands engage in a lectin interaction leading to the formation of a dense, crosslinked multimeric complex. On the other hand, vesicles with many ligands, or completely covered with them, switch the lectin interaction to form a fully soluble monomeric complex, without crosslinking. Nanomolar dissociation constants govern these interactions, as assessed by a ligand-displacement assay. The study demonstrates the switching nature – between monomeric and multimeric – of the interaction as a function of ligand density in the vesicles; the results are directly relevant to understanding such a phenomenon occurring at cell surfaces. |
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Keywords: | carbohydrates cell-surface mimics lectins polydiacetylene polymers vesicles |
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