Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction |
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Authors: | Dr Qian Wang Xinli Fan Nannan Jing Han Zhao Dr Lijia Yu Prof Xinjing Tang |
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Affiliation: | 1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, Beijing, P. R. China;2. National Center for Occupational Safety and Health, NHC, No. 27 Shilong Road, Beijing, P. R. China |
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Abstract: | Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes. |
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Keywords: | caged siRNAs host/guest interactions ligand modification photochemistry RNA interference |
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