Peptide-PAINT Enables Investigation of Endogenous Talin with Molecular Scale Resolution in Cells and Tissues |
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Authors: | Lisa S Fischer Dr Thomas Schlichthaerle Dr Anna Chrostek-Grashoff Prof?Dr Carsten Grashoff |
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Affiliation: | 1. Department of Quantitative Cell Biology, Institute of Molecular Cell Biology, University of Münster, Schlossplatz 5, Münster, 48149 Germany;2. Department of Biochemistry, University of Washington, Seattle, WA 98195 USA |
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Abstract: | Talin is a cell adhesion molecule that is indispensable for the development and function of multicellular organisms. Despite its central role for many cell biological processes, suitable methods to investigate the nanoscale organization of talin in its native environment are missing. Here, we overcome this limitation by combining single-molecule resolved PAINT (points accumulation in nanoscale topography) imaging with the IRIS (image reconstruction by integrating exchangeable single-molecule localization) approach, enabling the quantitative analysis of genetically unmodified talin molecules in cells. We demonstrate that a previously reported peptide can be utilized to specifically label the two major talin isoforms expressed in mammalian tissues with a localization precision of <10 nm. Our experiments show that the methodology performs equally well as state-of-the-art single-molecule localization techniques, and the first applications reveal a thus far undescribed cell adhesion structure in differentiating stem cells. Furthermore, we demonstrate the applicability of this peptide-PAINT technique to mouse tissues paving the way to single-protein imaging of endogenous talin proteins under physiologically relevant conditions. |
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Keywords: | cell adhesion peptides protein-protein interactions single-molecule studies talin |
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