Surface Density of Ligands Controls In-Plane and Aggregative Modes of Multivalent Glycovesicle-Lectin Recognitions |
| |
Authors: | Shivender Yadav Dr Kottari Naresh Prof?Dr Narayanaswamy Jayaraman |
| |
Affiliation: | 1. Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560012) India;2. Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560012) India
Present address: HP Green R&D Centre, KIADB Industrial Area, Bangalore, 560067 India |
| |
Abstract: | Glycovesicles are ideal tools to delineate finer mechanisms of the interactions at the biological cell membranes. Multivalency forms the basis which, in turn, should surpass more than one mechanism in order to maintain multiple roles that the ligand-lectin interactions encounter. Ligand densities hold a prime control to attenuate the interactions. In the present study, mannose trisaccharide interacting with a cognate receptor, namely, Con A, is assessed at the vesicle surface. Synthetic (1→3)(1→6)-branched mannose trisaccharides tethered with a diacetylene monomer and glycovesicles of varying sugar densities were prepared. The polydiacetylene vesicles were prepared by maintaining uniform lipid concentrations. The interactions of the glycovesicles with the lectin were probed through dynamic light scattering and UV-Vis spectroscopy techniques. Binding efficacies were assessed by surface plasmon resonance. Aggregative and in-plane modes of interactions show ligand-density dependence at the vesicle surface. Vesicles with sparsely populated ligands engage lectin in an aggregative mode (trans-), leading to a cross-linked complex formation. Whereas glycovesicles embedded with dense ligands engage lectin interaction in an in-plane mode intramolecularly (cis-). Sub-nanomolar dissociation constants govern the intramolecular interaction occurring within the plane of the vesicle, and are more efficacious than the aggregative intermolecular interactions. |
| |
Keywords: | carbohydrates cell surface mimics glycovesicles lectins molecular recognition multivalency polydiacetylene vesicles |
|
|