Regulating CRISPR/Cas9 Function through Conditional Guide RNA Control |
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| Authors: | Wes Brown Wenyuan Zhou Prof. Dr. Alexander Deiters |
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| Affiliation: | 1. Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260 USA These authors contributed equally to this work.;2. Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, PA 15260 USA |
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| Abstract: | Conditional control of CRISPR/Cas9 has been developed by using a variety of different approaches, many focusing on manipulation of the Cas9 protein itself. However, more recent strategies for governing CRISPR/Cas9 function are based on guide RNA (gRNA) modifications. They include control of gRNAs by light, small molecules, proteins, and oligonucleotides. These designs have unique advantages compared to other approaches and have allowed precise regulation of gene editing and transcription. Here, we discuss strategies for conditional control of gRNA function and compare effectiveness of these methods. |
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| Keywords: | Cas9 CRISPR gene editing nucleic acid optical control protection groups |
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