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Synthetic Phenolic Glycolipids for Application in Diagnostic Tests for Leprosy |
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| Authors: | J. Hessel M. van Dijk Anouk van Hooij L. Melanie Groot Jolijn Geboers Rosita Moretti Els Verhard-Seymonsbergen Danielle de Jong Gijs A. van der Marel Paul L. A. M. Corstjens Prof. Jeroen D. C. Codée Annemieke Geluk |
| Affiliation: | 1. Leiden Institute for Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden (The, Netherlands These authors contributed equally to this work.;2. Department of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden (The, Netherlands These authors contributed equally to this work.;3. Leiden Institute for Chemistry, Leiden University, Einsteinweg 55, 2333 CC Leiden (The, Netherlands;4. Department of Infectious Diseases, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden (The, Netherlands;5. Department Cell and Chemical Biology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden (The, Netherlands |
| Abstract: | Point-of-care (POC) diagnostic tests for the rapid detection of individuals infected with Mycobacterium leprae, the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinical outcome and reducing transmission of this infectious disease. Levels of antibodies directed against the M. leprae-specific phenolic glycolipid I (PGL−I) closely correlate with an individual's bacterial load and a higher risk of developing leprosy. We describe herein the assembly of a set of PGL glycans carrying the characteristic phenol aglycon and featuring different methylation patterns. The PGL trisaccharides were applied to construct neoglycoproteins that were used to detect anti-PGL IgM antibodies in leprosy patients. ELISAs and quantitative lateral-flow assays based on up-converting nanoparticles (UCP-LFAs) showed that the generated PGL−I and PGL-II trisaccharide neoglycoconjugates can be applied for the detection of anti M. leprae IgM antibodies in POC tests. |
| Keywords: | carbohydrates diagnosis glycoconjugates lateral flow assay leprosy |