首页 | 本学科首页   官方微博 | 高级检索  
     


Discovery and Engineering of a Novel Baeyer-Villiger Monooxygenase with High Normal Regioselectivity
Authors:Guang-Xiang Zhang  Zhi-Neng You  Jia-Mei Yu  Yuan-Yang Liu  Dr. Jiang Pan  Prof. Jian-He Xu  Dr. Chun-Xiu Li
Affiliation:State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237 P. R. China
Abstract:Baeyer-Villiger monooxygenases (BVMOs) are remarkable biocatalysts for the Baeyer-Villiger oxidation of ketones to generate esters or lactones. The regioselectivity of BVMOs is essential for determining the ratio of the two regioisomeric products (“normal” and “abnormal”) when catalyzing asymmetric ketone substrates. Starting from a known normal-preferring BVMO sequence from Pseudomonas putida KT2440 (PpBVMO), a novel BVMO from Gordonia sihwensis (GsBVMO) with higher normal regioselectivity (up to 97/3) was identified. Furthermore, protein engineering increased the specificity constant (kcat/KM) 8.9-fold to 484 s−1 mM−1 for 10-ketostearic acid derived from oleic acid. Consequently, by using the variant GsBVMOC308L as an efficient biocatalyst, 10-ketostearic acid was efficiently transformed into 9-(nonanoyloxy)nonanoic acid, with a space-time yield of 60.5 g L−1 d−1. This study showed that the mutant with higher regioselectivity and catalytic efficiency could be applied to prepare medium-chain ω-hydroxy fatty acids through biotransformation of long-chain aliphatic keto acids derived from renewable plant oils.
Keywords:Baeyer-Villiger monooxygenases  biocatalysis  oxidation  regioselectivity  renewable resources
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号