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Luminescent Amphiphilic Aminoglycoside Probes to Study Transfection |
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| Authors: | Alexander Zimmermann Qais Z. Jaber Dr. Johannes Koch Steffen Riebe Dr. Cecilia Vallet Dr. Kateryna Loza Matthias Hayduk Dr. Kfir B. Steinbuch Prof. Shirley K. Knauer Prof. Micha Fridman Jun.-Prof. Jens Voskuhl |
| Affiliation: | 1. Faculty of chemistry (Organic Chemistry) and, Centre for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitätsstrasse 7, 45117 Essen, Germany These authors authors contributed equally to this work.;2. School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 6997801 Israel;3. Center for Medical Biotechnology (ZMB), University of Duisburg Essen, Universitätsstrasse 2, 45141 Essen, Germany;4. Faculty of chemistry (Organic Chemistry) and, Centre for Nanointegration Duisburg-Essen (CENIDE), University of Duisburg-Essen, Universitätsstrasse 7, 45117 Essen, Germany;5. Institute for Molecular Biology, Centre for Medical Biotechnology (ZMB), University of Duisburg-Essen, Universitätsstrasse 2, 45117 Essen, Germany;6. Inorganic Chemistry and Centre for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitätsstrasse 7, 45141 Essen, Germany |
| Abstract: | We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation-induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ-potential and dynamic light-scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold-standard transfection reagent Lipofectamine® 2000. |
| Keywords: | aggregation-induced emission bioimaging cationic amphiphiles self-assembly transfection agents |