125I标价重组MIF在炎症模型小鼠体内的生物分布

摘要：目的:初步评价125I标记巨噬细胞移动抑制因子（MIF）在炎症显像中的应用价值。方法：Iodogen法制备125I-MIF，研究其稳定性、特异性及在炎症模型小鼠体内的生物学分布规律。结果：125I-MIF的标记率为96.5%，室温下48小时内标记化合物的生物学性质稳定。体内分布表明125I-MIF主要由肝脏代谢，经肾脏排泄，血液清除较快。尾静脉注射125I-MIF 0.5、1、6、24小时后，炎症肢体（靶）与对侧健肢（非靶）的%ID比值（T/NT）值分别为1.42、1.35、2.18和2.05。结论：125I-MIF具有在活体内炎症定位导向能力，但在早期优越性不明显，6小时后效果较佳，对隐匿性或亚急性炎症病灶的诊断有潜在的临床价值。

Bio-distribution of 125I labeled macrophage migration inhibitory factors in inflammatory model of mice

Abstract: Objective: To evaluate the usefulness of 125I labeled macrophage migration inhibitory factors (MIF) for the scintigraphic imaging of inflammation. Methods: 125I-MIF was radio-iodinated by Iodogen solid labeling method. The stability of 125I- MIF and its bio-distribution in inflammatory model of mice were studied. Results: The labeling rate of 125I- MIF was 96.5%. It was stable within 48 hours at room temperature. The bio-distribution results showed that the 125I- MIF was metabolized by the liver, the radioactivity clearance mainly happened in the kidney and the speed of the blood clearance is rapid. After caudal vein injection with 125I-MIF, the ratio of %ID between inflammatory limb (target) and contralateral healthy limb (non target)( T /NT) were 1.42, 1.35, 2.18 and 2.05 at 0.5, 1, 6, 24 hour respectively. Conclusion: 125I-MIF has the capability of locating the inflammatory foci. The advance of it is more obviously at the late stage than at the early stage. The 125I-MIF may be a potential agent for the diagnosis of concealed and subacute inflammatory disease.