| Abstract: | The aim of the study was to determine whether endogenous nitrogen and amino acid excretions at the terminal ileum change over time in the growing pig fed a protein‐free diet for 8 days. Seven entire male pigs with an overall mean live weight of 81.6 kg (SEM 3.3 kg) and surgically implanted post‐valve T caecum cannulas were fed a semi‐synthetic casein‐based diet for 8 days. Food was withheld from the pigs for 24 h, after which they were fed a protein‐free diet for a further 8 days at a rate of 10% of metabolic body weight per day. Chromic oxide was included in the protein‐free diet as an indigestible marker. Ileal digesta were collected continuously from 13:00 to 18:00 h on each day of the experimental period. Endogenous ileal nitrogen flows were determined for each pig each day the protein‐free diet was given, and endogenous ileal amino acid flows for the first and eighth days. There were no significant (P > 0.05) effects of the duration of feeding of the protein‐free diet on endogenous ileal total nitrogen or amino acid flows, except for the amino acids glycine and cysteine, the flows of which significantly decreased over the 8 day period (P < 0.01 and P < 0.05 for glycine and cysteine respectively), from (mean ± SEM) 1639 ± 217 to 892 ± 212 µg g?1 dry matter intake (DMI) for glycine and from 173 ± 13 to 127 ± 19 µg g?1 DMI for cysteine. The relative contributions (moles of each amino acid as a proportion of total moles of amino acids) of threonine, glycine and cysteine decreased significantly (P < 0.05) and that of proline increased significantly (P < 0.05) during the 8 days that the protein‐free diet was fed to the pigs. © 2000 Society of Chemical Industry |
