| 一株利用稻草秸秆发酵产油脂菌株的筛选及其发酵条件的初步研究 |
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| 引用本文： | 张鹏,姚日生,王淮,朱慧霞,邓胜松. 一株利用稻草秸秆发酵产油脂菌株的筛选及其发酵条件的初步研究[J]. 化工进展, 2012, 0(Z1): 86-90 |
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| 作者姓名： | 张鹏 姚日生 王淮 朱慧霞 邓胜松 |
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| 作者单位： | 合肥工业大学医学工程学院制药工程系;农产品生物化工教育部工程研究中心 |
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| 摘 要： | 从土壤中筛选到一株能在纤维素刚果红培养基上生长的真菌,经形态学和ITS全序列分析鉴定该菌株为长孢被孢霉PFY。菌株在发酵过程中不需额外添加纤维素酶就可直接利用预处理后的稻草秸秆进行发酵生产油脂。利用气质联用技术分析了所产油脂成分,该油脂中含有较多的不饱和组分,其中油酸和亚油酸百分含量为31.70%和27.30%。对其利用预处理后的秸秆进行发酵因素的考察,适宜发酵条件为底物浓度20g/L,初始pH6,温度28℃,接种量2%,添加0.2%KH2PO4和0.05%ZnSO4。在适宜条件下发酵后油脂得率为8.15%。
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| 关 键 词： | 微生物油脂 稻草秸秆 长孢被孢霉PFY 发酵 |
Screen of an oil-producing strain utilizing rice straw and its fermentation conditions |
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| ZHANG Peng,YAO Risheng,WANG Huai,ZHU Huixia,DENG Shengsong. Screen of an oil-producing strain utilizing rice straw and its fermentation conditions[J]. Chemical Industry and Engineering Progress, 2012, 0(Z1): 86-90 |
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| Authors: | ZHANG Peng YAO Risheng WANG Huai ZHU Huixia DENG Shengsong |
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| Affiliation: | 1,2(1School of Medical Engineering,Hefei University of Technology,Hefei 230009,Anhui,China;2Engineering Research Center of Bioprocess of Ministry of Education,Hefei University of Technology,Hefei 230009,Anhui,China) |
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| Abstract: | A novel fungi was isolated from soil by cellulose-congo red medium.It was identified as Mortierella elongata PFY by its morphological properties and internal transcribed spacer(ITS)sequence analysis.M.elongata PFY can produce oil utilizing pretreated rice straw without adding cellulase.The component of the lipid acids was analyzed by GC-MS and the content of oleic acid and linoleic acid were 31.70% and 27.30% respectively.The fermentation condition was:pretreated rice straw 20g/L,initial pH 6,inoculation 2%,0.2% KH2PO4,0.05% ZnSO4,28 ℃.At the suitable condition,the productivity of microbial oil was 8.15%. |
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| Keywords: | microbial oil rice straw Mortierella elongata PFY fermentation |
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