| ~(60)Co―γ射线诱变褐色高温单孢菌的研究 |
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| 引用本文： | 毛湘冰,肖兵南,刘毅,徐霞,杨爱芝,周望平.~(60)Co―γ射线诱变褐色高温单孢菌的研究[J].纤维素科学与技术,2006(1). |
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| 作者姓名： | 毛湘冰 肖兵南 刘毅 徐霞 杨爱芝 周望平 |
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| 作者单位： | 湖南农业大学动物科技学院，湖南省畜牧兽医研究所，湖南农业大学动物科技学院，湖南农业大学动物科技学院，湖南省宁乡县白马桥乡农技站，湖南省畜牧兽医研究所 湖南长沙410128，湖南省畜牧兽医研究所，湖南长沙，410131，湖南长沙410131，湖南长沙410128，湖南长沙410128，湖南宁乡410600，湖南长沙410131 |
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| 基金项目： | 2004年湖南省科技重大专项(04NK1006) |
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| 摘 要： | 以褐色高温单孢菌(Thermomonospora fusca)为出发菌株,通过60Co―γ射线诱变孢子悬液,采用透明圈法初筛和摇瓶培养复筛的方法,获得了一株纤维素酶高产菌株AV5,与出发菌株相比,其产酶能力提高1.8倍。
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| 关 键 词： | 褐色高温单孢菌 纤维素酶 ~(60)Co―γ射线诱变 |
Studies on Mutagenesis of Thermomonospora fusca with ~(60)Co―γ-Rays |
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| MAO Xiang-bing,XIAO Bing-nan,LIU Yi,XU Xia,YANG Ai-zhi,ZHOU Wang-ping.Studies on Mutagenesis of Thermomonospora fusca with ~(60)Co―γ-Rays[J].Journal of Cellulose Science and Technology,2006(1). |
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| Authors: | MAO Xiang-bing XIAO Bing-nan LIU Yi XU Xia YANG Ai-zhi ZHOU Wang-ping |
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| Affiliation: | MAO Xiang-bing1,2,XIAO Bing-nan2*,LIU Yi1,XU Xia1,YANG Ai-zhi3,ZHOU Wang-ping2 |
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| Abstract: | In this experiment,Thermomonospora fusca was chosen as an original strain for screening high yield cellulase mutants.The spore suspension of organism was treated with 60Co―γ-rays.A high cellulase-producing mutant strain AV5 was obtained by screening using the methods of transparent zone and shaking culture.The activity of mutant strain AV5 was enhanced for 1.8 times comparing with that of the original strain. |
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| Keywords: | Thermomonospora fusca cellulase mutantion of 60Co―γ-rays |
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