| 夜蓝荧光探针测定DNA的研究 |
| |
| 引用本文： | 武鑫,李生泉,张春杰.夜蓝荧光探针测定DNA的研究[J].分析仪器,2009(5):65-68. |
| |
| 作者姓名： | 武鑫 李生泉 张春杰 |
| |
| 作者单位： | 1. 山西农业大学文理学院,太谷,030801
2. 山西农业大学分析测试中心,太谷,030801 |
| |
| 摘 要： | 基于DNA对夜蓝（NB）荧光的增强作用，以二苯基萘基甲烷类碱性染料夜蓝为荧光探针，建立了一种新的DNA荧光测定体系。实验表明，在pH=6．4的K2HPO4-KH2PO4缓冲溶液中，当DNA的浓度在0．01～0．8μg／mL时，线性方程为：△F=627．85C＋6．2547（C：μg／mL），相关系数r=0．9986，检测限为4．2×10^-5μg／mL。该方法用于榛树嫩叶中DNA含量的测定，结果令人满意。
|
| 关 键 词： | 碱性染料 夜蓝(NB) 荧光探针 DNA |
Determination of DNA using night blue as fluorescence probe |
| |
| Wu Xin,Li Shengquan,Zhang Chunjie.Determination of DNA using night blue as fluorescence probe[J].Analytical Instrumentation,2009(5):65-68. |
| |
| Authors: | Wu Xin Li Shengquan Zhang Chunjie |
| |
| Affiliation: | Wu Xin1,Li Shengquan2,Zhang Chunjie2 (1. College of Arts and Sciences,Shanxi Agricultural University,Taigu,030801,2. Analysis and Test Center of Shanxi Agricultural University,030801) |
| |
| Abstract: | Based on the mechanism that DNA can enhance the intensity of night blue （NB） fluorescence, a new method has been developed for the determination of DNA using bisphenylnaphthlymethane dye night blue as fluorescence probe. In K2HPO4- HK2PO4 buffer solution （pH=6.4）, when DNA concentration was 0.01-0.8 μg/mL, the linear equation was △F = 627.85C＋6. 254, r =0. 9986, and the detection limit was 0.01μg/mL. The method has been successfully applied to the determination of DNA in hazel leaves. |
| |
| Keywords: | DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
